Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit
PCR kit iyi, yokonzeka kugwiritsidwa ntchito, imapezeka mumtundu wa lyophilized (njira yowumitsa zowuma) kuti isungidwe kwa nthawi yayitali.Chombocho chikhoza kunyamulidwa ndikusungidwa kutentha kwa chipinda ndipo chimakhala chokhazikika kwa chaka chimodzi.Chubu chilichonse cha premix chimakhala ndi ma reagents ofunikira pakukulitsa kwa PCR, kuphatikiza Reverse-transcriptase, Taq polymerase, primers, probes, ndi dNTPs substrates.Zimangofunika kuwonjezera madzi osungunuka a 13ul ndi template ya 5ul yotulutsidwa ya RNA, ndiye imatha kuthamanga ndikukulitsidwa pazida za PCR.
Makina a qPCR ayenera kukumanazofunika zotsatirazi:
1. Fit 8 strip PCR chubu voliyumu 0.2 ml
2. Khalani ndi njira zopitilira zinayi zozindikirira:
| Channel | Chisangalalo (nm) | Kutulutsa (nm) | Madyedwe Osasinthika |
| 1. | 470 | 525 | FAM, SYBR Green I |
| 2 | 523 | 564 | VIC, HEX, TET, JOE |
| 3. | 571 | 621 | ROX, TEXAS-RED |
| 4 | 630 | 670 | CY5 |
PCR-nsanja:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P
Kuvuta kwa mayendedwe ozizira unyolo waNovel Coronavirus nucleic acid kuzindikira reagent
Pamene ma reagents odziwika bwino a nucleic acid amanyamulidwa patali, (-20 ± 5) ℃ kusungirako ndi kuzizira kwa unyolo kumafunika kuti kuwonetsetsa kuti ma enzyme mu reagents amakhalabe achangu.Kuonetsetsa kuti kutentha kumafika pamlingo, ma kilogalamu angapo a ayezi wouma amafunikira pabokosi lililonse la nucleic acid test reagent ngakhale zosakwana 50g, koma zimatha masiku awiri kapena atatu okha.Potengera machitidwe amakampani, kulemera kwenikweni kwa ma reagents operekedwa ndi opanga ndi ochepera 10% (kapena ocheperako kuposa mtengo uwu) wa chidebecho.Zolemera zambiri zimachokera ku ayezi wouma, mapaketi a ayezi ndi mabokosi a thovu, kotero kuti mtengo wamayendedwe ndi wokwera kwambiri.
Mu Marichi 2020, COVID-19 idayamba kufalikira kumayiko ena, ndipo kufunikira kwa Novel Coronavirus nucleic acid kuzindikira reagent kudakula kwambiri.Ngakhale mtengo wamtengo wapatali wotumizira ma reagents mu unyolo wozizira, opanga ambiri amatha kuvomerezabe chifukwa cha kuchuluka kwakukulu komanso phindu lalikulu.
Komabe, ndi kusintha kwa mfundo za dziko zogulitsa zinthu zotsutsana ndi mliri, komanso kukweza ulamuliro wa dziko pa kayendetsedwe ka anthu ndi kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka kayendetsedwe ka anthu. ndi mayendedwe.Nthawi yowonjezereka yoyendera (nthawi yoyendera pafupifupi theka la mwezi ndiyofala kwambiri) imabweretsa kulephera kwazinthu pafupipafupi pomwe mankhwalawo afika kwa kasitomala.Izi zasokoneza mabizinesi ambiri ogulitsa ma nucleic acid.
Ukadaulo wa Lyophilized wa PCR reagent unathandizira mayendedwe aNovel Coronavirus nucleic acid kuzindikira reagent padziko lonse lapansi
The lyophilized PCR reagents akhoza kunyamulidwa ndi kusungidwa kutentha kwa firiji, zomwe sizingangochepetsa mtengo wa mayendedwe, komanso kupewa mavuto omwe amayamba chifukwa cha kayendetsedwe ka kayendetsedwe ka kayendetsedwe kake.Choncho, lyophilizing reagent ndiyo njira yabwino yothetsera vuto la kayendedwe ka kunja.
Lyophilization imaphatikizapo kuziziritsa njira kuti ikhale yolimba, kenako ndikusiyanitsa nthunzi wamadzi pansi pa vacuum.The solute zouma amakhala mu chidebe ndi zikuchokera chimodzimodzi ndi ntchito.Poyerekeza ndi ma reagents ochiritsira amadzimadzi, gawo lonse la lyophilized Novel Coronavirus nucleic acid detective reagent yopangidwa ndi Liming Bio ili ndi izi:
Kukhazikika kwa kutentha kwambiri:imatha ndi chithandizo choyima pa 56 ℃ kwa masiku 60, ndipo mawonekedwe ndi magwiridwe antchito a reagent amakhalabe osasintha.
Kusungirako kutentha kwanthawi zonse ndi mayendedwe:palibe chifukwa cha unyolo wozizira, palibe chifukwa chosungira kutentha kochepa musanamasule, kumasula kwathunthu malo osungira ozizira.
Zakonzeka kugwiritsidwa ntchito:lyophilizing onse zigawo zikuluzikulu, palibe chifukwa dongosolo kasinthidwe, kupewa imfa ya zigawo zikuluzikulu ndi mkulu mamasukidwe akayendedwe monga enzyme.
Multiplex targets mu chubu chimodzi:chandamale chodziwikiratu chimakhudza jini ya coronavirus ya ORF1ab, jini ya N, jini ya S kupewa kufalikira kwa kachilomboka.Pofuna kuchepetsa zolakwika zabodza, jini ya RNase P yaumunthu imagwiritsidwa ntchito ngati chiwongolero chamkati, kuti akwaniritse zosowa zachipatala zowongolera khalidwe lachitsanzo.
