Novel Coronavirus (2019-nCoV) Ribonucleic Acid Detection Kit (Real-time PCR - Fluorescent Probe Assay)
ZolingaGwiritsani ntchito
Izi zidapangidwa kuti zizindikirike bwino za novel coronavirus (SARS-CoV-2) mu zitsanzo za kupuma.Zotsatira zozindikiridwa zitha kugwiritsidwa ntchito kuthandiza kuzindikira matenda a Coronavirus 2019 (COVID-19).
Zigawo
2019-nCoV PCR Buffer - mayeso 96
PCR Enzyme Mix - 96 mayeso
2019-nCoV Positive Control - mayeso 48
2019-nCoV Negative Control - mayeso 48
2019-nCoV Internal Control - mayeso 96
Phukusi Lowetsani - 1 kopi
ZogulitsaNjira
Izi zili ndi mapeyala anayi a PCR primers, ma probes anayi a fulorosenti, RNA reverse transcriptase, DNA polymerase, dNTP, magnesium ion, ndi mankhwala ena ozindikira ma virus a RNA.Imayang'ana jini ya RNA polymerase (RdRp) yodalira RNA, Nucleocapsid (N) jini, ndi Envelop (E) jini ya 2019-nCoV nthawi imodzi mu chubu chimodzi choyesera.Njira yoyesera imayenda motsatira njira zitatu izi: 1) Reverse-transcribe viral RNA mu viral DNA.2) Kukulitsa ma virus a DNA kuti athe kuyeza.3) Nenani kuchuluka kwa ma amplicons a DNA kudzera mu kafukufuku wosakanizidwa.Kupatula apo, mankhwalawa amaphatikizanso zowongolera zamkati ndi zowongolera zakunja kuti ziwunikire momwe zimagwirira ntchito pamayendedwe ndi kusungirako.
